Composition of parental mitochondrial DNA in cloned bovine embryos
Abstract
We have investigated parental mitochondrial DNA (mtDNA) in cloned bovine embryos obtained by intraspecific cytoplast-blastomere fusion. Analysis of two-cell to blastocyst stage embryos revealed that in contrast to the exclusion of paternal (sperm) mtDNA during sexual inheritance in the cytoplast-blastomere fusion complexes, there was mixing and co-existence of parental mtDNA. The mixing of mtDNA was non-balanced with the minority deriving from the blastomere. The constant content of mtDNA during embryogenesis until the blastocyst stage suggesting an absence of mtDNA replication was shown for conventional `in vitro fertilised' (IVF) embryos and for cloned embryos. The ratio of parental mtDNA was in accordance with the estimated quantitative participation of mtDNA from the fusion partners.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1998
- DOI:
- Bibcode:
- 1998FEBSL.426..352S
- Keywords:
-
- Cloning;
- Bovine;
- Mitochondrial DNA;
- Fusion;
- Allele-specific quantitation;
- TaqMan™ polymerase chain reaction;
- DB;
- donor blastomere;
- RC;
- recipient cytoplast;
- CR;
- control region;
- AS-PCR;
- allele-specific PCR;
- NTC;
- no template control;
- NAC;
- no amplification control;
- T a;
- annealing temperature;
- PIRA;
- primer introduced restriction analysis